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Image Search Results
Journal: Skeletal Muscle
Article Title: A novel tetracycline-responsive transgenic mouse strain for skeletal muscle-specific gene expression
doi: 10.1186/s13395-018-0181-y
Figure Lengend Snippet: A schematic of the HSA-rtTA transgene. The promoter and first exon (− 2,000 to + 239 relative to the transcription start site) of the human skeletal muscle α-actin (HSA) gene regulates expression of an optimized reverse tetracycline transactivator (rtTA) gene which has been reported to be sevenfold more active and 100-fold more doxycycline sensitive than the original Tet-On system . The β-globin intron ΙΙ (BGI) and poly(A) tail were incorporated into the transgene to ensure proper splicing and transcript stability, respectively. The positions of the PCR primers used for genotyping are indicated by half-arrows
Article Snippet: As previously described by us for the HSA-MerCreMer transgene, the promoter and first exon (− 2,000 to + 239 relative to the transcription start site) of the human
Techniques: Expressing